Strain variation among Bordetella pertussis isolates circulating in Poland , after 50 years 1 of whole - cell pertussis vaccine use 2

15 In this study, clinical isolates of Bordetella pertussis collected in Poland from 1960 to 16 2005 were analyzed by pulsed-field gel electrophoresis (PFGE) according to recommended 17 references. Among the 110 isolates from 1995-2005, 59 PFGE patterns were found, most of 18 which were different from those currently circulating in other EU countries for which data are 19 available. PFGE patterns of currently disseminating B. pertussis clones were found within 20 PFGE groups III and IV, as elsewhere in the EU, and in newly identified clusters A and C. Up 21 to 70%, 26%, and 4%, respectively, of the currently isolated strains in Poland harbored 22 ptxA1/prn1, ptxA1/prn2, ptxA1/prn3 allele combinations and most (82%) were found to be of 23 the Fim2 phenotype. Differences in the extent of heterogeneity estimated by PFGE typing in 24 B. pertussis populations circulating in Poland in comparison to other EU countries may be 25 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.01487-10 JCM Accepts, published online ahead of print on 9 February 2011 on O cber 1, 2017 by gest ht://jcm .sm .rg/ D ow nladed fom